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首页> 外文OA文献 >Genome-Wide Screening and Identification of Factors Affecting the Biosynthesis of Prodigiosin by Hahella chejuensis, Using Escherichia coli as a Surrogate Host ▿ †
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Genome-Wide Screening and Identification of Factors Affecting the Biosynthesis of Prodigiosin by Hahella chejuensis, Using Escherichia coli as a Surrogate Host ▿ †

机译：使用大肠杆菌作为替代宿主，全基因组筛选和鉴定影响Hahella chejuensis的Prodigiosin生物合成的因素††

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摘要

A marine bacterium, Hahella chejuensis, recently has attracted attention due to its lytic activity against a red-tide dinoflagellate. The algicidal function originates from its red pigment, prodigiosin, which also exhibits immunosuppressive or anticancer activity. Genome sequencing and functional analysis revealed a gene set contained in the hap gene cluster that is responsible for the biosynthesis of prodigiosin. To screen for the factors affecting the prodigiosin biosynthesis, we constructed a plasmid library of the H. chejuensis genomic DNA, introduced it into Escherichia coli strains harboring the hap cluster, and observed changes in production of the red pigment. Among the screened clones, hapXY genes whose products constitute a two-component signal transduction system were elucidated as positive regulators of the pigment production. In addition, an Hfq-dependent, noncoding region located at one end of the hap cluster was confirmed to play roles in regulation. Identification of factors involved in the regulation of prodigiosin biosynthesis should help in understanding how the prodigiosin-biosynthetic pathway is organized and controlled and also aid in modulating the overexpression of prodigiosin in a heterologous host, such as E. coli, or in the natural producer, H. chejuensis.
机译：海洋细菌Hahella chejuensis最近因其对赤潮甲藻的分解活性而受到关注。杀藻功能源自其红色色素prodigiosin，后者还具有免疫抑制或抗癌活性。基因组测序和功能分析揭示了hap基因簇中包含的一个基因组，负责prodigiosin的生物合成。为了筛选影响prodigiosin生物合成的因素，我们构建了H. chejuensis基因组DNA的质粒文库，将其引入具有hap簇的大肠杆菌菌株中，并观察了红色素生成的变化。在筛选出的克隆中，阐明了其产物构成两组分信号转导系统的hapXY基因作为色素生成的正向调节剂。另外，证实位于hap簇的一端的依赖于Hfq的非编码区在调控中起作用。鉴定参与调节洋地黄菌素生物合成的因素应有助于理解如何组织和控制地洋地黄菌素生物合成途径，还有助于调节异源宿主（例如大肠杆菌）或天然生产者中地黄菌素的过度表达， H. chejuensis。

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